Inhibition of liver cancer cell line by hydatid cyst fluid

Worm parasites, including Echinococcus granulosus, contain compounds that exhibit antitumor activity. So, the objective of this study was to evaluate the effects of antigen B extracted from hydatid cyst fluid on liver Hep G۲Methods: Effects of different concentrations of antigen B on Hep G۲ and HEK۲۹۳ cells proliferation were investigated using the MTT assay. Cell cycle analysis to measure cellular DNA content in the G۰/G۱, S and G۲/M was done using Flow cytometry. Annexin-V/PI staining method was used to determine cells apoptotic rate. Furthermore, the mRNA expression of pro-apoptotic gene BAX and anti-apoptotic gene BCL۲ was assessed by RT-PCR after exposure to antigen B.Results: The effect of antigen B on HEK۲۹۳ and Hep G۲ cells showed that HEK۲۹۳ cells as a normal cell line is less sensitive than cancer cell line B۱۶F۱۰ to antigen B, and IC۵۰ values in HEK۲۹۳ and Hep G۲cells were ۳۵ ± ۴.۳ and ۱۵ ± ۳.۱ μM, respectively. In both cell lines, the antigen B induced anti-proliferative effect on the cells with increasing cell population at G۰/G۱, and decreasing the numbers of cells at the S and G۲/M phases. Our results also showed antigen B can induce cell apoptosis in both HEK۲۹۳ and B۱۶F۱۰ cell lines and increased the mRNA expression of BAX and decreased the mRNA expression of BCL۲ thereby induction of cell apoptosis.Conclusion: this study confirmed that antigen B inhibits proliferation and promotes apoptosis of HEK۲۹۳ and Hep G۲cells and can raise hopes in the treatment of melanoma cancesupplement in the toxicity caused by rice tablets.