The Effect of settled bed height and bed expansion on hydrodynamic behavior of expanded bed adsorption for protein purification

Fluidized/Expanded bed adsorption (EBA) has received increasing attentions as an effective technique for the purification of proteins directly from cell homogenates or fermentation broths in downstream processing .To widely extend the EBA technology in biological industries, better understanding of the expanded-bed behavior is necessary to maximize its efficient applications .In this study streamline-DEAE adsorbent was loaded into 1.6cm and 1cm column and comparison of liquid phase mixing with different settled bed height(SBH) (SBH=5,6,7,8,9,10,11,12cm) and different bed expansion (30%,80%, 130%,180%),respectively ,for obtaining the optimal SBH and bed expansion has been carried out. Hydrodynamic characteristics (based on RTD experiments )of 9 cm for SBH and 130% for bed expansion demonstrated a minimal mixing which is desirable for Macromolecular and Nanoparticle purification. The generic effect of settled bed height and bed expansion and subsequently bioseperation performance in such integrated processes is fully discussed.

Keywords:

Expanded bed adsorption , fluidized bed , downstream processing , settled bed height , bed expansion , integrated process