Expression of galectin-8 on human endometrium: Molecular and cellular aspects

Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
View: 476

This Paper With 6 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_IJRM-11-1_010

تاریخ نمایه سازی: 16 شهریور 1395

Abstract:

Background: The up-regulation of galectin-3, galectin-9, and galectin-15 expression in the luminal and glandular epithelium was reported in preparation ofthe endometrium for embryo implantation at the midlutheal phase. However, no data was available regarding the expression and the distribution pattern of galectin-8 inthe human endometrium during a regular menstrual cycle. Objective: The current study designed to investigate the expression and thedistribution pattern of galectin-8, a beta-galactoside-binding lectin in the human endometrium during both proliferative and luteal phases of a regular menstrualcycle. Materials and Methods: Endometrial biopsies were obtained from the anterior wall of the uterine cavity of 16 women (proliferative phase: n=4, lutheal phase: n=12).All female patients with mean age of 37.5 years were fertile (range 25-45). Each biopsy was divided into three pieces; one piece was fixed in formaldehyde for light microscopy and immunohistochemistry. The second portion fixed in glutaraldehydefor scanning electron microscopy and the third portion was prepared for western blot analysis. Results: Data of immunoblotting revealed a molecular weight of 34 kD band withhigh intensity in the lutheal phase samples. The immunohistochemistry stainingdemonstrated that galectin-8 expressed at a very low concentration during the proliferative phase, but showed a high expression throughout the lutheal phase. Theexpression of galectin-8 observed in luminal surface epithelium, glandular epithelium and stroma. Conclusion: The up-regulation of the expression of galectin-8 during lutheal phasemay suggest galectin-8 as one of the potential molecular marker of the endometrial receptivity. These data propose that galectin-8 may play an important role during the initial events of human embryo implantation

Authors

Hossein Nikzad

Anatomical Sciences Research Center, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran

Hamed Haddad Kashani

Anatomical Sciences Research Center, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran

Maryam Kabir-Salmani

Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

Yoshihiro Akimoto

Department of Anatomy, School of Medicine, Kyorin University, Mitaka, Tokyo, Japan