Plasmid DNA Transformation in Agrobacterium Tumefaciens, LBA 4404 by Standard Method Cacl2 / Heat Shock

A pulse of 30 second duration combined with 37°C heat shock temperature exhibited maximum efficiency of transformation as revealed by kanamycin resistant colonies appearing on LB Agar kanamycin plates. When the cells were further incubated on ice for 10 min, the transformation efficiency increased compared to heat shock treatment. These results suggest that a heat shock pulse of 30 sec at 37°C followed by a 10 min ice incubation step are ideal parameters to obtain maximum Plasmid DNA transformation efficiency in LBA4404 strain.

Keywords:

Transformation , Agrobacterium tumefaciens , Plasmid , CaCl2 , Heat Shock