Evaluation effect of purslaneon rat liver necrosis using FTIR spectroscopy technique

FTIR spectroscopy is a technique to identify structural changes of macromolecules such as carbohydrates, protein, DNA and lipids.Study was undertaken toevaluate the effects of purslane on copper sulphate poisoning on liver structure changes. Thus, twenty eight wistar rats were randomly allocated to four treatment groups. Group I) Control, Group II) Copper sulphate (200mg/kg bodyweight was applied bygavage daily for four week, Group III) Purslane (gavage400 mg/kg bodyweight daily for four weeks) and Group IV) Combined treatment of copper sulphateand purslane as described in Group II and III. At the end of the study, animals were sacrificed and the liver was removed for Fourier transform infrared spectroscopy (FTIR). We confirmedthat purslane administration protected the carbohydrate structural damage that occurred in liver of copper treated rats

Keywords:

Purslane , FTIR Spectra , Copper sulphate