Vitamin C prevention of anemia-induced changes in structural and functional parameters of mice prostate

Background: Phenylhydrazine (PHZ) as a well-known compound causes toxicity in different levels in tissues. The present study aimed to evaluate the probable protective effects of vitamin C against the tissue damages caused by PHZ in mice ventral prostate.Methods: Twenty-eight adult mice were divided randomly into four groups: first group received 0.1 ml of normal saline intraperitoneally; second group received 60 mg/kg of phenylhydrazine in 48-hour intervals intraperitoneally; third group received 60 mg/kg of phenylhydrazine along with 250 mg/kg of vitamin C intraperitoneally and fourth group received the same doses of vitamin C like the third group. After 35 days, formalin fixed ventral prostate was processed using standard histological method. Paraffin blocks were sectioned at 5–6 μm and stained with Hematoxylin and Eosin (H&E), Periodic Acid Schiff (PAS), Masson’s trichrome.Result: the results of prostate tissue section staining using H&E, PAS and Masson’s trichrome revealed positive effects of prescription of vitamin C on anemia-induced changes in histological structure of ventral prostate. The high densities of secretory alveoli was considerable in groups received vitamin C. Furthermore, vitamin C caused an increase in folded secretory alveoli in the periphery of ventral prostate. Stromal connective tissue was increased between the secretory alveoli in vitamin C groups. Toxic effects of PHZ were seen through the production of reactive oxygen species (ROS) and inhibition of ventral prostate activity.Conclusion: It seems that vitamin C as free radical inhibitors decreased the ventral prostate toxicity induced by PHZ in mice.

Keywords:

Histochemical , Mice , Ventral Prostate , Vitamin C , Phenylhydrazine