Detection of H2O2 and glucose using peroxidase mimicking activity of CuO/GFs aerogel

In this research, CuO nanoparticles decorated on graphenebased frameworks (CuO/GFs) were prepared by hydrothermal method. The structure, composition, andmorphology of the prepared nanocomposite were characterized using X‐ray diffraction and scanning electron microscopy with energy dispersive X‐ray spectroscopy, respectively. A colorimetric system involving nanozyme, 3,3´,5,5´-tetramethylbenzidine (TMB) and H2O2 was utilized for the determination of peroxidase-like catalytic assay. Also, The Michaelis– Menten constants (Km) and maximum initial velocities (Vmax) of CuO/GFs was calculated using Lineweaver– Burk plot. The obtained results confirmed that the synthesis of CuO/GFs nanostructures was successful. It was found that CuO/GFs nanohybrid exhibited peroxidase mimetic activity. Peroxidase - like activity was highest at a temperature of 30° Can dp H3.0 but was moderately active at a temperature of 20-60°C. Also, a combination of the peroxidase‐like catalytic activity of the CuO/GFs nanohybrid with glucose oxidase (GOX) allowed the system of a sensitive colorimetric assay for glucose monitoring.

Keywords:

CuO/GFs aerogel , Peroxidase-like activity , Characterization , Tetramethylbenzidine