Identification of genes involved in herbal docetaxel-resistant prostate cancer

Docetaxel is an anti-cancer drug used to treat various cancers, including breast, lung and prostate metastasis. Docetaxel, by attaching to the microtubules, inhibits the mitosis and thus leads to slow growth and release of cancer cells. The purpose of this research is to identify the genes involved in prostate cancer, to investigate biological processes and to identify the genes involved in key pathways. In this study, data on GSE47040 were analyzed by Flex Array software and performed by T-test. The analyzed data was filtered with Fold change (> 2, <-2). Then, based on the abbreviation of the genes, biological processes were involved and key pathways were identified. Based on the analysis, 68 genes in treating with docetaxel in the resistant cell line (TaxR) increased expression versus the sensitive cell line (C4-2B) and 72 genes reduced expression. By investigating the gene ontology of identified genes, involved processes of biology included: cellular process, response to stimuli, metabolic process, immune process and biology (increased), and locative and reproductive processes (reduced). Key genes identified in different pathways included YWHAH, BCL3, CCL4L1, and PRKCA.

Keywords:

Gene ontology , Docetaxel , Metastases prostate cancer , TaxR and C4-2B Cell lines