Somatic embryogenesis induction from hypocotyl explants of Catharanthus roseus (L.) G. Don

The current research was conducted to simulate the efficient in vitro somatic embryogenesis of Periwinkle Catharanthus roseus. 1 mg/l 2,4-D+0/5 mg/l BAP significantly affected the calli fresh weight. 1 and 0/5 mg/l 2,4-D concentration had meaningful effect on the production of pre-embryonic tissue and subsequent embryogenesis. With the second experiment, radicle, hypocotyl and leaf explants from in vitro derived plants were employed. For callogenesis, the interaction effects of radicle × 2,4-D, hypocotyl × 2,4-D and radicle × NAA were significant. For calli fresh weight, hypocotyl × NAA and hypocotyl × 1mg/l NAA+ 1mg/l BAP were the treatments of choice. For pre-embryonic tissue and the embryos produced, hypocotyl × 2, 4-D was the best treatment.

Keywords:

Catharanthus roseus , Plant growth regulators , Somatic embryogenesis , MS medium