Purification of laccase from newly isolated Bacillus Safensis and analysis of biochemical and structural properties of Laccase enzymes

laccase (EC 1.10.3.2) is an extracellular oxygenated oxidizer that uses molecular oxygen as the final receptor. In this study, the purity and location of the laccase enzyme from the new Bacillus safensisbacteria. The laccase active protein, expression was performed under aerobic conditions and at low temperature. The recombinant protein by the column tends to customize and determine the molecular gel of the enzyme by 60 SDS-PAGE clients. The enzyme has the best performance at 35 degree. The optimum pH of the enzyme activity is 5.5. This distinguishes its unusual property from other known bacterial laccases.

Keywords:

Bacillus safensis , laccase , expression , aerobic conditions , recombinant