The gene expression patterns of stromal cells in prostate tissue during prostate cancer

Introduction: The development of stromal cells in prostatic glands revealed that these cells not only nourishand support the secretory epithelium of the glands, but also contribute to the morphogenesis, its hormonalmetabolism and secretory function. It has also been exhibited that they are involved in the onset anddevelopment of cancer by producing reactive stroma. Accordingly, this study aims to analyze the geneexpression patterns of stromal cells in prostate gland during prostate cancer in comparison to normal tissue.Methods: The information related to normal prostate tissue was obtained by GEO database with GSE ۲۶۹۱۰and the stromal cells’ data was provided by GSE ۳۵۳۷۳. Four sets of data were gained for each group andthe platform used for both was [HG-U۱۳۳_Plus_۲] Affymetrix Human Genome U۱۳۳ Plus ۲.۰ Array. Dataanalysis was performed by R software. After entering the commands related to directory setting, showingmicroarray images, drawing histogram, data were normalized. Afterwards, heatmap commands were typedin order to observe the gene expression patterns.Results and discussion: The results of data analysis and heatmap illustration showed that there was asignificant difference between the gene expression patterns of stroma and normal cells which indicates theimportance of these cells in tumorigenesis. Given the substantial difference in the gene expression patternsof stromal cells and normal tissue as well as the complexity of prostate cancer treatment, it seems that moreattention to these cells could be an important therapeutic goal.

Keywords:

stromal cells , prostate cancer , gene expression , prostate gland , R software