A Decrease of Reelin Expression in Neonatal Ventral Hippocampal Lesion Model of Schizophrenia

Schizophrenia affects 1% of population. Neonatal ventral hippocampus lesion (NVHL) model of schizophrenia designed in 1993 by Lipska is a widely studied developmental animal model of schizophrenia. NVHL rats mimic many of the symptoms of schizophrenia in detail. We studied this model in molecular leveland reelin expression in it. Reelin is an extracellular matrix glycoprotein that regulates some processes in CNS development and reduces significantly inschizophrenia. For this study, animals (male pups) take into 3 groups: control, sham and experiment. The lesion made by injection of 0.3 μl. Isotonic Neurotoxin withstereotaxic surgery in age 7 day and body weight 11-15 gr. Social behavioral andstereotypic movement assessed in age 56 day then reelin expression in frontal cortex was evaluated by western blotting. Behavioral analysis and histological studiesdemonstrated the schizophrenia model. Western blotting of reelin protein in frontal cortex and hippocampus showed a decrease of reelin (P value: 0.012) inexperimental group as compared to control and sham group. So, in the NVHL as a common and more similar model of schizophrenia reelin expression significantly decreases in frontal cortex and hippocampus that means this model in molecular pathways is similar to the disease.

Keywords:

Schizophrenia , Expression , Reelin Activity