Conformation and dynamic of versatile peroxidase in the presence of the glycerol based deep eutectic solvent

Ionic liquids are considered as green solvents and promising candidates to replace organic solvents. A subclass of ionic liquids called deep eutectic solvents are synthesized by mixing a quaternary ammonium salt, the most conventional among them choline chloride, and a hydrogen bond donor such as glycerol. The deep eutectic solvents are simple to synthesize, biodegradable, and biocompatible. On the other hand, versatile peroxidase, capable of degrading a wide range of substrates high or low potential substrates, has attracted attention to be applied in industry. In the present study, the effect of hydrated choline chloride:glycerol on the conformation and dynamic of versatile peroxidase were studied using extrinsic fluorescence, steady-state anisotropy, and fluorescence lifetime measurements. According to the results of extrinsic fluorescence studies, presence of choline chloride:glycerol caused higher exposure of hydrophobic patches of versatile peroxidase. Furthermore, decreased values of steady-state anisotropy in the presence of increasing concentrations of choline chloride:glycerol revealed formation of versatile peroxidase assemblies. Moreover, higher fluorescence lifetime values in the presence of growing concentrations of the choline chloride:glycerol implied adoption of more compact conformation and consequently less flexibility of versatile peroxidase. In general, it could be concluded that hydrated choline chloride:glycerol can alter versatile peroxidase‟s conformation and dynamic.

Keywords:

Choline chloride:glycerol , Deep eutectic solvents , Versatile peroxidase , Conformation , Dynamic