Effect of lactate administration and physical exercise training on hippocampal neurogenesis in adult male rats
Publish place: 8th basic and clinical neuroscience congress
Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
View: 507
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
NSCMED08_059
تاریخ نمایه سازی: 15 دی 1398
Abstract:
Background and Aim : Physical exercise has been known to promote cognitive function and neurogenesis in adult rat brain. Although precise underlying mechanisms are still unknown, systemic exercise-induced signals proposed to be involved. Recently, lactate has been considered as a signaling molecule within the brain tissue. We aimed to investigate the effect of exogenous lactate administration as a potential exercise mimetic on hippocampal neurogenesis.Methods : Ault male Wistar rats were subjected to two weeks (5days/wk) of moderate-intensity exercise training consisted of treadmill running (15m/min, 60min/day) or daily injection of sodium-L-lactate (2gr/kg, IP). Sedentary animals used as the control group. Immunohistochemistry for doublecortin (DCX- a marker of developing immature neurons) and 5-bromo-2 -deoxyuridine (BrdU- a marker of proliferation) was performed to evaluate hippocampal neurogenesis.Results : Immunohistochemical analysis revealed that compared to the sedentary control animals, treadmill exercise training increases remarkably the number of BrdU- and DCX-positive cells in dentate gyrus of hippocampus. Administration of exogenous lactate increases immunoreactivity to DCX in compare to control sedentary group; however, BrdU immunoreativity remained unaffected in lactate group.Conclusion : Our results highlighted the possible role of systemic lactate as a mediator of exercise training effect on hippocampal neurogenesis. Future experimental investigation using lactate receptor blockade are warranted.
Keywords:
Authors
Zahra Akbari
Department of Physiology, Faculty of Medicine, Bushehr University of Medical Sciences, Iran
Mehrzad Sedaghat
Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran
Banafsheh Esmailzadeh
Department of Physiology, Faculty of Medicine, Bushehr University of Medical Sciences, Iran
Mohammad Reza Farzaneh
Department of Pathologyy, Faculty of Medicine, Bushehr University of Medical Sciences, Iran
Zeinab Mahdian
Department of Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Iran
Khalil Pourkhalili
Department of Physiology, Faculty of Medicine, Bushehr University of Medical Sciences, Iran