Investigation of changes in the expression of RNA-helicase (MOV10L1) gene in transgenic embryonic stem cells of rat exposed to retinoic acid

The Moloney leukemia virus 10-like 1 (Mov10l1) gene is specifically expressed in germ cells. Changes in the expression of this gene in presence of retinoic acid in the ESCs have been investigated. We conducted an experimental study to evaluate the Mov10l1 variation in transgenic OCT4-GFP ESCs (Gift from Max-Planck- Institute) when incubated at 5 and 10 μM retinoic acid. Time dependency at 7, 14 & 21th days was also figured out. SPSS and Excel software were used for data analysis. By comparing the CT of MOV10L1 gene with the housekeeping gene (HPRT), results indicated that the gene expressed 301 times than ESC in all experimental groups. The expression rate of the gene in the 5 μm group over the three-week period was higher than the 10 μm group. Various studies showed that; retinoic acid is a potential inducer for differentiation of stem cells. It can differentiate ESC into the neuron, germ like cell and other cells, depending on the concentration and exposure time. According to scientific sources, the corresponding protein of this gene has been observed only in testicular tissue and mRNA is expressed mainly in testicular tissue and less in epididymal, endometrial and bile ducts. Therefore, based on the results, it could be expected the germ cells and reproductive-related tissues in the experimental conditions.

Keywords:

Stem Cells , Mov10l1 , Retinoic acid